Thesis Abstract and Dissertation Abstracts
Molecular Genetic Diversity of Red Junglefowl (Gallus gallus) In Northern India And Its Admixture With Domestic Chicken
Abstract Category: Science
Course / Degree: DOCTORATE OF PHILOSOPHY
Institution / University: KURUKSHETRA UNIVERSITY, KURUKSHETRA, HARYANA, India
Published in: 2011
Genetic diversity, relationship and admixture analysis of Red Junglefowl (Gallus gallus) with domestic chicken in Northern India was assessed using 26 microsatellite markers. In total, 250 samples (230 Red Junglefowl and 20 domestic chickens) were collected across five Red Junglefowl range states in Northern India. In total, 541 alleles were observed across five Red Junglefowl and one domestic chicken population. The mean observed heterozygosity was lowest (Ho = 0.3512) in Uttarakhand Red Junglefowl population and highest (Ho = 0.5696) in Uttar Pradesh Red Junglefowl population. Locus LEI0234 yielded the highest (Na = 41) and locus MCW0103 yielded the lowest number of alleles (Na = 9). Nei’s genetic distance indicated that the Delhi Zoo and Uttarakhand Red Junglefowl populations are least similar or most distant (DA= 0.6032), while the Himachal Pradesh and Uttar Pradesh Red Junglefowl populations are most identical or least genetically distant (DA = 0.2501). The neighboring joining tree based on Nei’s and Chord distance showed two clear nodes: one in between Haryana and Delhi Zoo Red Junglefowl populations and other between Uttarakhand Red Junglefowl population and pooled domestic chicken population. The tree showed a close relationship between Uttar Pradesh and Himachal Pradesh Red Junglefowl population. The population assignment test showed Red Junglefowl of Haryana (Morni breeding centre) was relatively pure while samples of Uttarakhand, Uttar Pradesh and Delhi Zoo Red Junglefowl populations were scattered and inter-mixed, suggesting the continuity/gene flow in these populations. The correspondence analysis revealed that the Red Junglefowl samples of Himachal Pradesh and domestic chicken formed separate clusters while there was a mixing in the samples of Uttarakhand, Uttar Pradesh and Delhi Zoo. The minimum population differentiation or maximum gene flow was between Red Junglefowl of Uttar Pradesh and Himachal Pradesh (Nm 5.695) while maximum population differentiation or minimum gene flow was between Red Junglefowl of Delhi Zoo and Uttarakhand Red Junglefowl population (Nm 1.675). The overall, Nm values were quite high, suggesting the high gene flow and less genetic differentiation among Red Junglefowl of Northern India. The free gene flow also reflected genetic homogeneity and lack of population genetic structuring among Red Junglefowl populations in Northern India. The bottleneck analysis of captive stocks revealed a normal ‘L’ shaped distribution of allele frequencies and therefore, did not reveal the presence of any recent bottleneck in the recent past.
In addition, 15 microsatellite loci were also developed for Himalayan monal (Lophophorus impejanus) through cross species amplification of chicken microsatellites. These markers were among those used in the present study for red junglefowl genotyping. These markers could be employed in population genetic studies for Himalayan monal which is listed in schedule I of the Indian Wildlife (Protection) Act and listed in appendix I of the CITES.
Thesis Keywords/Search Tags:
RED JUNGLEFOWL, GENETIC DIVERSITY, RELATIONSHIP AND BOTTLENECK ANALYSIS
This Thesis Abstract may be cited as follows:
Mukesh (2011). Molecular Genetic Diversity of Red Junglefowl (Gallus gallus) in Northern India and its admixture with domestic chicken. Ph.D Thesis. Kurukshetra University, Kurukshetra, Haryana, 165 pp.
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The Red Junglefowl (click to enlarge) |
Submission Details: Thesis Abstract submitted by Mukesh Thakur from India on 11-Jun-2011 20:48.
Abstract has been viewed 3894 times (since 7 Mar 2010).
Mukesh Thakur Contact Details: Email: thamukesh@gmail.com Phone: +91-9358081705
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