Thesis Abstract and Dissertation Abstracts
PCR Primer designs to detect polymorphisms within leihmania vector Phlebotomus papatasi sand fly
Abstract Category: Science
Course / Degree: n/a
Institution / University: Al-Quds University, Jerusalem, Palestine
Published in: 2000
Phlebotomine sand flies are weak fliers with maximum dispersal seldom exceeds one kilometer away from their breeding sites. These flies are inactive in wind speeds over 4 kilometers per second and begin to seek shelter live in light winds. Geographic isolation among populations may promote genetic divergence resulting from genetic drift and /or selection for adaptive to regional habitats. For Phlebotomus papatasi, which has a broad geographical distribution, there is a good chance that the local populations are genetically isolated. Some proteins and enzymes in sand fly saliva are known to aid in the degradation of carbohydrates while others cause local erythemas at the bite site. These components prevent hemostasis, thereby facilitating the location of blood vessels in the host skin and maintaining an adequate blood supply. The saliva of some sand flies was shown to enhance the development of cutaneous leishmaniasis lesions as well. We have selected one of these proteins; α-amylase, to study species polymorphisms. The α- amylase gene from P. papatasi was cloned, sequenced and compared to Lutzomyia longipalpis. DNA was extracted from single P. papatasi male sand flies originating in different geographical locations in the West Bank. Four different upper and lower primers were designed and cross-tested against each other. Primers AU 489 and AL 997 were found efficient in detecting polymorphisms.At optimal annealing temperature the primers targeted sand fly DNA producing multiple PCR products and DNA polymorphisms were detected by comparing the banding profiles of different populations. 12 colony and field populations were studied and all of them gave different profiles characteristic to each population. Primers were tested on other sand flies including L.longipalpis as well as different species of Leishmania. We found that both Leishmania parasites and L. longipalpis produce profiles different from those of P. papatasi. The studies of the distribution of polymorphisms will provide description of the genetic structure of sand fly populations in order to improve our understanding of the role of the vector in the epidemiology of leishmaniasis through modulating the pathology of the parasite it transmits.
Thesis Keywords/Search Tags:
Phlebotomus papatasi, PCR, Primer design, leishmania, sand flies
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Submission Details: Thesis Abstract submitted by Omar Hamarsheh from Palestine on 05-Sep-2004 14:31.
Abstract has been viewed 2896 times (since 7 Mar 2010).
Omar Hamarsheh Contact Details: Email: ohamarsheh@med.alquds.edu
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